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mouse positive control primer set hoxc10  (Active Motif)


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    Active Motif mouse positive control primer set hoxc10
    Primers used in ChIP-qPCR for quality control. The designed primers were ordered from Integrated DNA Technologies, Coralville, IA, USA.
    Mouse Positive Control Primer Set Hoxc10, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+positive+control+primer+set+hoxc10/mouse+positive+control+primer+set+hoxc10/pmc07364495-348-0-6
    Average 90 stars, based on 1 article reviews
    mouse positive control primer set hoxc10 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications"

    Article Title: MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications

    Journal: Nature protocols

    doi: 10.1038/s41596-019-0223-x

    Primers used in ChIP-qPCR for quality control. The designed primers were ordered from Integrated DNA Technologies, Coralville, IA, USA.
    Figure Legend Snippet: Primers used in ChIP-qPCR for quality control. The designed primers were ordered from Integrated DNA Technologies, Coralville, IA, USA.

    Techniques Used: Control

    Optimization of antibody quantity using MOWChIP-qPCR for 4 histone marks. For each histone mark, 3 different quantities of antibody were used during IP bead coating and enrichment of ChIP DNA at positive (Hoxc10 for H3K27me3 and H3K9me3; Actb-1 for H3K36me3 and H3K79me2) and negative (Neg Set 3 for H3K27me3 and H3K9me3; Neg Set 1 for H3K36me3 and H3K79me2) loci. Enrichment was examined by qPCR under each condition. All experiments were conducted in duplicate and the horizontal lines represent the mean. The relative fold enrichment was normalized against that of Neg Set 3 or Neg Set 1. 1000 nuclei from mouse prefrontal cortex were used in each assay and MNase digestion was used for chromatin fragmentation. 10-week old CD-1 male mice were used in the study. The nuclei were not sorted and included neurons and glia. The animal protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of Virginia Commonwealth University.
    Figure Legend Snippet: Optimization of antibody quantity using MOWChIP-qPCR for 4 histone marks. For each histone mark, 3 different quantities of antibody were used during IP bead coating and enrichment of ChIP DNA at positive (Hoxc10 for H3K27me3 and H3K9me3; Actb-1 for H3K36me3 and H3K79me2) and negative (Neg Set 3 for H3K27me3 and H3K9me3; Neg Set 1 for H3K36me3 and H3K79me2) loci. Enrichment was examined by qPCR under each condition. All experiments were conducted in duplicate and the horizontal lines represent the mean. The relative fold enrichment was normalized against that of Neg Set 3 or Neg Set 1. 1000 nuclei from mouse prefrontal cortex were used in each assay and MNase digestion was used for chromatin fragmentation. 10-week old CD-1 male mice were used in the study. The nuclei were not sorted and included neurons and glia. The animal protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of Virginia Commonwealth University.

    Techniques Used:

    Related Articles

    Positive Control:

    Article Title: MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications
    Article Snippet: .. Mouse Positive Control Primer Set Hoxc10 (Active Motif, cat. no. 71019). ..

    other:

    Article Title: MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications
    Article Snippet: ● Silicon wafer (3-inch diameter; University Wafer, cat. no. P(100)1-100ohm-cm SSP, test grade) ● Petri dish (10-cm; Fisher Scientific, cat. no. 08-757-100D) ● Eppendorf microcentrifuge tube (1.5-ml; VWR, cat. no. 20170-038) ● Spin coater (Laurell, model no. WS-400BZ-6NPP/LITE) ● Hot plate (Thermo Fisher Scientific, model no. HP8885794) ● Vacuum pump (Welch Vacuum, model no. WOB-L 2522B-01) ● Precleaned glass slide (VWR, cat. no. 48300-026) ● UV lamp (OmniCure, series no. 1000) ● Sonicator (focused ultrasonicator; Covaris, model no. M220) ● PDMS hole puncher (Harris, cat. no. 69036-20) ● Plasma cleaner (Harrick Plasma, cat. no. PDC-32G) ● Vacuum pump (Dekker Vacuum Technologies, model no. RVR003H-01) ● Thread seal tape (Teflon tape, 1/2 inch × 520 inches; NDA Distributors, item no. M3) ● Pressure gauges (McMaster-Carr, cat. nos.



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    mouse positive control primer set hoxc10  (Active Motif)
    90
    Active Motif mouse positive control primer set hoxc10
    Primers used in ChIP-qPCR for quality control. The designed primers were ordered from Integrated DNA Technologies, Coralville, IA, USA.
    Mouse Positive Control Primer Set Hoxc10, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+positive+control+primer+set+hoxc10/mouse+positive+control+primer+set+hoxc10/pmc07364495-348-0-6
    Average 90 stars, based on 1 article reviews
    mouse positive control primer set hoxc10 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Primers used in ChIP-qPCR for quality control. The designed primers were ordered from Integrated DNA Technologies, Coralville, IA, USA.

    Journal: Nature protocols

    Article Title: MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications

    doi: 10.1038/s41596-019-0223-x

    Figure Lengend Snippet: Primers used in ChIP-qPCR for quality control. The designed primers were ordered from Integrated DNA Technologies, Coralville, IA, USA.

    Article Snippet: Mouse Positive Control Primer Set Hoxc10 (Active Motif, cat. no. 71019).

    Techniques: Control

    Optimization of antibody quantity using MOWChIP-qPCR for 4 histone marks. For each histone mark, 3 different quantities of antibody were used during IP bead coating and enrichment of ChIP DNA at positive (Hoxc10 for H3K27me3 and H3K9me3; Actb-1 for H3K36me3 and H3K79me2) and negative (Neg Set 3 for H3K27me3 and H3K9me3; Neg Set 1 for H3K36me3 and H3K79me2) loci. Enrichment was examined by qPCR under each condition. All experiments were conducted in duplicate and the horizontal lines represent the mean. The relative fold enrichment was normalized against that of Neg Set 3 or Neg Set 1. 1000 nuclei from mouse prefrontal cortex were used in each assay and MNase digestion was used for chromatin fragmentation. 10-week old CD-1 male mice were used in the study. The nuclei were not sorted and included neurons and glia. The animal protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of Virginia Commonwealth University.

    Journal: Nature protocols

    Article Title: MOWChIP-seq for low-input and multiplexed profiling of genome-wide histone modifications

    doi: 10.1038/s41596-019-0223-x

    Figure Lengend Snippet: Optimization of antibody quantity using MOWChIP-qPCR for 4 histone marks. For each histone mark, 3 different quantities of antibody were used during IP bead coating and enrichment of ChIP DNA at positive (Hoxc10 for H3K27me3 and H3K9me3; Actb-1 for H3K36me3 and H3K79me2) and negative (Neg Set 3 for H3K27me3 and H3K9me3; Neg Set 1 for H3K36me3 and H3K79me2) loci. Enrichment was examined by qPCR under each condition. All experiments were conducted in duplicate and the horizontal lines represent the mean. The relative fold enrichment was normalized against that of Neg Set 3 or Neg Set 1. 1000 nuclei from mouse prefrontal cortex were used in each assay and MNase digestion was used for chromatin fragmentation. 10-week old CD-1 male mice were used in the study. The nuclei were not sorted and included neurons and glia. The animal protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of Virginia Commonwealth University.

    Article Snippet: Mouse Positive Control Primer Set Hoxc10 (Active Motif, cat. no. 71019).

    Techniques: